Phosphate buffer is an economical non-protonated buffer, and there also are a variety of perdeuterated buffers (d-Tris, d-HEPES) available that do not obscure protein NMR signals. Protein preparation ...

A pressure of 3,000 bar is applied to the cold shock protein B of Bacillus subtilis in a small tube in the NMR spectroscopy laboratory at the University of Konstanz. This is roughly three times ...

Structure determination by NMR typically requires a protein concentration of 0.5 mM or greater, stable for several days at the desired temperature, usually 20-40 o C. Proteins and peptides should be ...

To capture the ensembles of conformations adopted by disordered proteins, researchers needed a method that was better suited to their twists and turns. Nuclear magnetic resonance (NMR) spectroscopy ...

Researchers try multiple means to get high-quality membrane proteins for X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy studies. As much as 30% of human genes code for ...

With a new method for optimizing the amino acid isotopic labeling pattern, stereo-array isotope labeling (SAIL), structures of large proteins (up to 50 kDa) can now be solved with ease with ...